Dab tsi yog cov qauv rau kev ua electrophoretic?

Dab tsi yog cov qauv rau kev ua electrophoretic?

Lub hauv paus ntsiab lus ntawm electrophoresis yog hais txog cov txheej txheem ntawm cov khoom siv hluav taws xob tsiv teb tsaws nyob rau hauv qhov kev txiav txim ntawm hluav taws xob teb. Ntau yam biomolecules tseem ceeb, xws li amino acids, peptides, proteins, nucleotides, nucleic acids, thiab lwm yam, muaj ionizable pawg uas tuaj yeem nqa cov nqi zoo lossis tsis zoo ntawm tus nqi pH. Nyob rau hauv qhov kev txiav txim ntawm ib qho hluav taws xob teb, cov nqi molecules yuav txav mus rau lub electrode kev taw qhia opposite rau lub polarity ntawm lawv cov nqi.

Electrophoretic txuj ci yog lub peev xwm los cais, txiav txim siab lossis ua kom huv cov qauv los ntawm kev siv hluav taws xob, uas ua rau cov khoom siv hluav taws xob txav mus los ntawm qhov sib txawv ntawm qhov sib txawv vim qhov sib txawv ntawm cov khoom siv hluav taws xob ntawm ntau yam molecules hauv cov qauv sib cais, nrog rau qhov sib txawv. nyob rau hauv qhov loj, cov duab thiab lwm yam khoom ntawm cov molecules lawv tus kheej. Cov txheej txheem electrophoresis yuav tsum tau ua nyob rau hauv ib qho kev txhawb nqa nruab nrab. Qhov dawb interface electrophoresis ua los ntawm Tiselius li al. Xyoo 1937 tsis muaj qhov nruab nrab txhawb nqa, yog li dispersion thiab convection kuj muaj zog, cuam tshuam rau kev sib cais. Yog li nws nthuav tawm electrophoresis nrog ib qho kev txhawb nqa ruaj khov, qhov twg cov qauv ua tiav cov txheej txheem electrophoresis hauv nruab nrab, txo qhov cuam tshuam ntawm dispersion thiab convection.

Cov xov xwm txhawb nqa thawj zaug yog cov ntawv lim dej thiab cov cellulose acetate membrane, uas tam sim no tsis tshua siv hauv cov chaw kuaj mob. Tau ntev, cov khoom siv me me xws li amino acids, peptides, qab zib, thiab lwm yam feem ntau tau sib cais thiab tshuaj xyuas los ntawm electrophoresis siv cov ntawv lim los yog cellulose lossis silica gel nyias txheej daim hlau raws li kev tshaj tawm. Txawm li cas los xij, tam sim no, cov txheej txheem nquag siv xws li HPLC feem ntau yog siv los tshuaj xyuas. Cov xov xwm no tsim nyog rau kev sib cais cov khoom me me, nrog kev ua haujlwm yooj yim thiab yooj yim. Txawm li cas los xij, kev sib cais ntawm cov kab mob biomolecules tsis zoo yog qhov tsis zoo. Kev taw qhia ntawm gel ua ib qho kev txhawb nqa nruab nrab tau txhawb nqa kev txhim kho ntawm cov txuj ci electrophoresis, ua rau cov txuj ci electrophoresis dhau los ua ib qho tseem ceeb hauv kev txheeb xyuas cov macromolecules lom xws li cov protein thiab nucleic acids.

Electrophoretic processing tsis yooj yim, tab sis peb kuj yuav tsum paub tias muaj qee cov qauv rau kev ua electrophoretic, txwv tsis pub peb tsis paub tias qhov zoo yog tsim nyog. Yog li dab tsi yog cov qauv rau kev ua electrophoretic no?

1. Smooth nto

Qhov no yog ib qho kev mob yooj yim, yog tias qhov saum npoo tsis du, ces qhov kev tsim nyog tau ploj lawm;

2. Cov tuab tuab

Electrophoretic processing yuav tsum ua kom cov tuab tuab los tiv thaiv cov xim sib txawv ntawm qhov tshwm sim;

3. Tsis muaj qhov pom tseeb tawg ntawm qhov chaw